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I get an error code 18 in the plot view when I opened my bam files

asked 2013-01-02 19:35:20 -0600

Rose Ismet gravatar image

I get an error code 18 in the plot view when I opened my bam files. Please help. Is it my bam files? The file was aligned using the CASAVA software.

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answered 2013-01-03 10:35:39 -0600

Hi Rose,

I am sorry you are having issues loading your files into GenomeBrowse.

Error code 18 is reported when GenomeBrowse is unable to recognize and/or find a current reference sequence for the build associated with the BAM file.

We have seen this particular error occur because of genome build incompatibility. Does your BAM file contain human data? If so what reference sequence was used to sequence your files? The available reference sequence for human build hg19 in GenomeBrowse was created from data provided by UCSC if CASAVA uses a different source, for example 1000 Genome data, that could be the issue.

We would be happy to help you identify exactly what is happening but would need for you to provide us with a sample of your data. Please email us at genomebrowse@goldenhelix.com if you would be will to share your files in a secure fashion.

Thanks, Jami...

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Asked: 2013-01-02 19:35:20 -0600

Seen: 3,390 times

Last updated: Jan 03 '13