asked 2017-04-26 05:27:54 -0600
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Dear all, Maybe this is a naive question but I want to ask you what theshould you use in read depth to consider that the variant may indeed be worth for further study (sanger sequence)and it's not a false result?
I read about 20, I don't remember where, but I'm not sure if is a good limit or not. Thank you
Hi Rute,
For Sanger sequencing typically you would look at the Chromatograms. Here is a nice webpage that provides additional details.
https://seqcore.brcf.med.umich.edu/si...
If you goal is to view a chromatogram to view ab files and assess SNP calls from Sanger sequencing, there are a variety of free tools (genestudio, Sequence Scanner, 4 peaks, etc…).
Kind Regards,
-Steve
Questions should be tagged FeatureRequest for asking about a non-existing feature or proposing a new idea, GeneralInquiry for general questions about GenomeBrowse or directions on how to do something, or RanIntoProblem if you want to report an issue or had difficulty getting to an expected result.
Asked: 2017-04-26 05:27:54 -0600
Seen: 1,073 times
Last updated: Aug 09 '17
