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How can I estimate the linkage disequilibrium from my imputed data set?

Hi everyone, I'm new to this software and to this forum, I would need to estimate the linkage disequilibrium using my data. I have around 3000 individuals with a list of SNP genotyped and imputed. I would like to create a LD plot from my population. I would like to do this on a small region of around 100kb. my file is in this format:

Subjects          SNP1      SNP2        SNP3     SNP4
A01               0.02       0          0.5      ...
A02               0.45       1          0.57     ...
A03               1.6        2          0.3      ...
....              ....       ...        ...      ...

Any suggestion?

Thanks