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READ DEPTH TRESHOULD

asked 2017-04-26 05:27:54 -0600

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Dear all, Maybe this is a naive question but I want to ask you what theshould you use in read depth to consider that the variant may indeed be worth for further study (sanger sequence)and it's not a false result?

I read about 20, I don't remember where, but I'm not sure if is a good limit or not. Thank you

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answered 2017-08-09 12:02:13 -0600

SteveHystad gravatar image

Hi Rute,

For Sanger sequencing typically you would look at the Chromatograms. Here is a nice webpage that provides additional details.

https://seqcore.brcf.med.umich.edu/si...

If you goal is to view a chromatogram to view ab files and assess SNP calls from Sanger sequencing, there are a variety of free tools (genestudio, Sequence Scanner, 4 peaks, etc…).

Kind Regards,

-Steve

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Asked: 2017-04-26 05:27:54 -0600

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Last updated: Aug 09