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Hi Whit,

Are you looking at a reference sequence file or a gene annotation file? Both forward and reverse strand information is available for any reference sequence plot just by increasing the vertical height of the graph. It would look something like the below figure, where the top information is for the forward strand and the bottom information is the reverse strand at this location.

If you are looking at a gene annotation file then the strand for a gene is fixed based on strand field listed in the source of the annotation information.

Any gene listed on the forward strand will be colored blue and any gene on the reverse strand will be green.

Let me know if you have any further questions.

Thanks, Jami...